Journal: Analytical Chemistry

Article Title: Early Detection of Multiple Sclerosis Using Spectroscopic Epstein–Barr Virus Biosensors Based on Infection Mechanism Mimicry

doi: 10.1021/acs.analchem.5c03964

Figure Lengend Snippet: SEM (A–C) and AFM (D–F) images of (A, D) CD21@AuNP, (B,E) BSA@CD21@AuNP, and (C, F) gp350@BSA@CD21@AuNP.

Article Snippet: Recombinant human CD21 and recombinant EBV gp350 proteins were purchased from Sino Biological Inc. All reagents and chemicals employed in this study were analytical grade and used without further purification.

Techniques:

Journal: Analytical Chemistry

Article Title: Early Detection of Multiple Sclerosis Using Spectroscopic Epstein–Barr Virus Biosensors Based on Infection Mechanism Mimicry

doi: 10.1021/acs.analchem.5c03964

Figure Lengend Snippet: (A) FTIR and (B) UV spectra for the characterization of the developed colorimetric EBV biosensor. Step-by-step FTIR and UV spectra of the biosensor during gp350 detection: (a) 2.1 mL of AuNPs; (b) 2.1 mL of AuNPs + 400 μL of CD21 at 20 ng·mL –1 ; (c) 2.1 mL of AuNPs + 400 μL of CD21 at 20 ng·mL –1 + 100 μL of 1% BSA; and (d) 2.1 mL of AuNPs + 400 μL of CD21 at 20 ng·mL –1 + 100 μL of 1% BSA + 50 ng·mL –1 of gp350.

Article Snippet: Recombinant human CD21 and recombinant EBV gp350 proteins were purchased from Sino Biological Inc. All reagents and chemicals employed in this study were analytical grade and used without further purification.

Techniques:

Journal: Analytical Chemistry

Article Title: Early Detection of Multiple Sclerosis Using Spectroscopic Epstein–Barr Virus Biosensors Based on Infection Mechanism Mimicry

doi: 10.1021/acs.analchem.5c03964

Figure Lengend Snippet: (A) UV spectra and (B) bar chart that belong to the optimization of the CD21–gp350 interaction incubation temperature. (a) BSA@CD21@AuNP, (b) BSA@CD21@AuNP + 50 ng·mL –1 gp350 for 4 °C, (c) 25 °C, (d) 37 °C, and (e) 45 °C incubation temperature.

Article Snippet: Recombinant human CD21 and recombinant EBV gp350 proteins were purchased from Sino Biological Inc. All reagents and chemicals employed in this study were analytical grade and used without further purification.

Techniques: Incubation

Journal: Analytical Chemistry

Article Title: Early Detection of Multiple Sclerosis Using Spectroscopic Epstein–Barr Virus Biosensors Based on Infection Mechanism Mimicry

doi: 10.1021/acs.analchem.5c03964

Figure Lengend Snippet: (A) UV spectra and (B) Excel plot that belong to the optimization of the CD21–gp350 interaction incubation time. (a) BSA@CD21@AuNP, (b) BSA@CD21@AuNP + 50 ng·mL –1 gp350 for 15 min, (c) 30 min, (d) 60 min, and (e) 90 min incubation time.

Article Snippet: Recombinant human CD21 and recombinant EBV gp350 proteins were purchased from Sino Biological Inc. All reagents and chemicals employed in this study were analytical grade and used without further purification.

Techniques: Incubation

Journal: Analytical Chemistry

Article Title: Early Detection of Multiple Sclerosis Using Spectroscopic Epstein–Barr Virus Biosensors Based on Infection Mechanism Mimicry

doi: 10.1021/acs.analchem.5c03964

Figure Lengend Snippet: Analytical characteristics of the developed colorimetric EBV biosensors. (A) UV spectra of (a) BSA@CD21@AuNP, (b) 10 ng·mL –1 , (c) 25 ng·mL –1 , (d) 50 ng·mL –1 , (e) 80 ng·mL –1 , and (f) 100 ng·mL –1 gp350 additions. (B) Calibration plot of increasing concentrations of gp350 from 10 ng·mL –1 to 100 ng·mL –1 .

Article Snippet: Recombinant human CD21 and recombinant EBV gp350 proteins were purchased from Sino Biological Inc. All reagents and chemicals employed in this study were analytical grade and used without further purification.

Techniques:

Journal: Analytical Chemistry

Article Title: Early Detection of Multiple Sclerosis Using Spectroscopic Epstein–Barr Virus Biosensors Based on Infection Mechanism Mimicry

doi: 10.1021/acs.analchem.5c03964

Figure Lengend Snippet: UV spectra demonstrating the absorbance difference of a 1:1 ratio of (a) BSA@CD21@AuNP, (b) gp350 @BSA@CD21@AuNP, and (c) A27L + KMP11 + gp350 @BSA@CD21@AuNP.

Article Snippet: Recombinant human CD21 and recombinant EBV gp350 proteins were purchased from Sino Biological Inc. All reagents and chemicals employed in this study were analytical grade and used without further purification.

Techniques: